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Image Search Results
Journal: Scientific Reports
Article Title: Integrin targeting of glyphosate and its cell adhesion modulation effects on osteoblastic MC3T3-E1 cells revealed by label-free optical biosensing
doi: 10.1038/s41598-018-36081-0
Figure Lengend Snippet: Competitive αvβ3 integrin binding assay with ( a ) ELISA ( b ) Epic BT: Solutions of integrin were co-incubated with the corresponding compounds (glyphosate, echistatin, tirofiban) in the RGD-coated wells of the ELISA or biosensor microplates, and integrin binding to the RGD motifs on the surface was detected. Signals obtained were corrected for the non-specific background (assay signal without integrin or compound addition) and were normalised for assay signals obtained with uninhibited integrin (control). Data are shown as a mean ± SD of at least triplicates (n = 3–6). Statistical analysis was performed with the Graph Pad Prism program. Asterisks indicate significant differences from the control (p < 0.0001).
Article Snippet: The biosensor measurements were performed using 96- or 384-well
Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Control